1. DC subset cooperation for activation of antiviral T cells.
DC subset cooperation for activation of antiviral T cells. (A) Stimulation of an SL9-specific CD8+ T cell line by HLA-A2− CD1c+ DCs mixed with HLA-A2+ CD141+ DCs after infection with HIV-1, outline of the experiment. (B) GFP expression in CD1c+ and CD141+ DCs and frequency of CD8+ T cells positive for TNF and IFN-γ expression 48 hours after infection with GFP-encoding HIVSL9(G) Vpx (MOI = 1) (n = 3 combined from two experiments). (C) Stimulation of M1- and NS1-specific CD8+ T cell lines by HLA-A2− DCs mixed with HLA-A2+ CD141+ DCs after infection with influenza virus, outline of the experiment. (D) GFP expression in mixed CD1c+ and CD141+ DCs 4 hours after infection with GFP-tagged FluA(PR8). (E) IFN-γ concentration in culture supernatants of M1- and NS1-specific CD8+ T cell lines exposed for 18 hours to DCs 12 hours after infection with GFP-tagged FluA(PR8) (MOI = 2; n = 3 combined from two experiments). **P < 0.01, ***P < 0.001, ****P <
Aymeric Silvin et al. Sci. Immunol. 2017;2:eaai8071
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