1. EVPP 110 Molecules & Processes of Life
Activities:
Microscopy
Water Properties / Alteration by Pollutants
Week of February 5th, 2018

2. Group photo
© Xiatong Liu
“Frozen Raindrops”
© Tina Le

3. Reminders
Each person must submit their own Weekly Data Sheets and Weekly Write-Ups.
Even though the WDSs are done as a group, I cannot assign you a grade unless you upload the file to BlackBoard from your own account.
Please include your full name, my name and the section number on ALL pages submitted to BlackBoard.
BlackBoard does not organize the submissions by instructor and I want to make sure I see your assignment.

4. Molecules & Process of Life
Activity 1 – Microscopy

5. Compound Microscope Tutorial:
Eyepiece
Compound Microscope
Neck
Nosepiece
Objectives
Stage controls
Macrofocus
Stage
Diaphragm
Tutorial:
Light source
Microfocus
Base
Light adjustment

6. Example of type of image seen with compound microscope.

7. How to prepare a wet mount:
Cover slip
Lower slowly

8. Observe prepared letter “e” slide (Fig 1.1).
Prepare stained yeast culture slide (Fig 1.2).
Unaided eye
Compound microscope
Unaided eye
Compound microscope
Figure 1.1. Sketch of prepared letter “e” slide as viewed with unaided eye and compound microscope.
Figure 1.2. Sketch of yeast culture, stained with crystal violet, as viewed with the unaided eye and compound microscope.

9. Prepare algae culture slide (Fig 1.3).
Prepare wet mount of underside of plant leaf (Fig 1.4).
Unaided eye
Compound microscope
Unaided eye
Compound microscope
Figure 1.3. Sketch of algae culture, unstained, viewed with unaided eye and compound microscope.
Figure 1.4. Sketch of thin section of plant leaf underside as viewed with unaided eye and compound microscope.

10. Dissecting microscope
Eyepiece
Dissecting microscope
Zoom
Objective
Focus
Upper light
Lower light

11. Example of type of image seen with dissecting microscope.

12. Observe whole plant leaf (Fig 1.5). Observe whole insect (Fig. 1.6).
Unaided eye
Dissecting microscope
Unaided eye
Dissecting microscope
Figure 1.5. Sketch of whole plant leaf viewed with unaided eye and dissecting microscope.
Figure 1.6. Sketch of invertebrate viewed with unaided eye and dissecting microscope.

13. Observe whole rock (Fig. 1.7).
Observe whole bracket fungus (Fig. 1.8).
Unaided eye
Dissecting microscope
Unaided eye
Dissecting microscope
Figure 1.7. Sketch of whole rock viewed with unaided eye and dissecting microscope.
Figure 1.8. Sketch of whole bracket fungus viewed with unaided eye and dissecting microscope.

14. Molecules & Process of Life
Activity 2 – Properties of Water and Alteration by Pollutants

15. Water properties demonstrated:
Fill 140 mL plastic container with tap water (unpolluted) until bulging.
Predict number of 1.25 mL spoons of powdered sugar that can be added to the water before it spills.
Add sugar one spoon at a time until water spills.
Repeat procedure with simulated polluted water.
Table 2.1
Unpolluted Water
Polluted Water
Part A.
Predicted
Actual
# of 1.25 mL spoons of powdered sugar added to 140 mL container of water before water spills:
Explanation:
Water properties demonstrated:

16. Water properties demonstrated:
Fill one balloon with tap water.
Fill 2nd balloon with air (from pump) until same size as 1st balloon.
Predict number seconds it will take for air balloon to pop (Table 2.2).
Hold air balloon over candle, record number of seconds until it pops.
Repeat procedure (including prediction) with water balloon.
Table 2.2
Part B.
Prediction
Actual
# seconds until air balloon pops after contact with flame:
Explanation:
# seconds until water balloon pops after contact with flame:
Water properties demonstrated:

17. Water properties demonstrated:
Predict and record number drops of unpolluted water that can be placed on a penny before the water spills over (Table 2.3).
Count actual number of water drops record your results.
Repeat procedure (including prediction) with simulated polluted water.
Table 2.3
Unpolluted Water
Polluted Water
Part C.
Prediction
Actual
# water drops that can be placed on a penny before the water spills over:
Explanation:
Water properties demonstrated:

18. Water properties demonstrated:
Fill weigh boat with tap water until water is level with (but not bulging above) rim.
Gently place sewing pin on water surface.
Observe and record appearance of water surface adjacent to pin (Table 2.4).
Table 2.4
Part D.
Prior to Pollutant
After Pollutant
Appearance of water surface adjacent to sewing pin:
Behavior of the sewing pin on/in water:
Explanation of behavior:
Water properties demonstrated:
Dip tip of a toothpick in liquid soap (pollutant), touch soapy tip to water surface and observe what happens.
Record observations (Table 2.4).

19. What’s Due Weekly Data Sheets: Weekly Write-Ups:
PowerPoint available at:
OK to turn in hard copy
Post to BlackBoard