1. DNA fingerprinting (genetic fingerprint)
Introduction and work instruction about the procedure of the experiment
DNA-Fingerprint

2. Content 1. DNA fingerprinting introduction 1.1 Areas of application
1.2 Comparison classic and modern procedure
1.3 DNA marker source
1.4 Restriction enzymes
DNA-Fingerprint

3. 1.1 Areas of Application Forensic identifying perpetrators
e.g. with blood/ skin cells, hair, sperm
Proof of foreign genes:
- in Organisms (are they transgene?)
- in grocery (Are hamburgers made out of pure beef?
Discovery of a biological father or mother
Proof if twins are genetic identical
DNA-Fingerprint

4. 1.2 Comparison Classic procedure Modern procedure
Introns
Minisatellites VNTRs bp
Restriction
Gelelectrophoresis
Blotting
Hybridisation with VNTR-sond
Introns
Microsatellites STR bp
PCR
Gelelectrophoresis
DNA-Fingerprint

5. Advantages of a modern procedure
DNA-areas are shorter with max bp (vs bp)
PCR is cheaper and faster (1 day)
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6. Investigated DNA-Sections
Intron 1 CCTATTA 5 times repeated
Exon
Intron 2 without CCTATTA
Exon
Intron 3 CCTATTA 10 times repeated
Repititive sequences in the introns are individual different.
Sequenz polymorphisms come up through differences at one single basepair  one sequence of recognition gets lost  different pattern of bands in the gel
DNA-Fingerprint

7. 1.3 DNA Marker Source: Lambda-Phage
hosted by E. coli
Genom Bp (1982 sequenced)
Genom is cuted by restriction enzyme Eco RI
head
tale
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8. 1.4 Restriction Enzymes cuts sticky ends C T T A A G
EcoR G A A T T C
cuts sticky ends C T T A A G
Pst1 C T G C A G
(Providencia stuartii) G A C G T C
- HaeIII G G C C cuts blunt ends C C G G
DNA-Fingerprint