1. Optical imaging to assess metabolic changes in kidney transplant animal model
Students: Farnaz H.Foomani, Soudeh Mostaghimi, Shima Mehrvar
Advisor: Dr. Mahsa Ranji
Collaborator: Dr. Allen Cowley, Department of Physiology, MCW
04/29/2019

2. Motivation Kidney transplant can be modeled by Uninephrectomy (UNX).
Unx would result to the nephron hypertrophy.
Nephron hypertrophy contributes to structural and functional changes to the remaining kidney.

3. Experimental Protocol
Day 0
Day 3
No kidney extraction
Both kidneys extraction
Left kidney extraction
Right kidney extraction (UNX)

4. 3D Fluorescence Cryoimager
To detect autofluorescence signals of NADH and FAD
To calculate redox state NADH/FAD as an index for oxidative stress

5. Result and Conclusion
Volumetric Redox Ratio
Lower redox ratio (blue color) is related to higher oxidative stress.
This study helps to find effective mitochondrial-targeted clinical interventions.
As a model for transplantation it can help to treat hypertrophied kidney .

6. Acknowledgment Support: Collaborator: Biophotonics lab:
Dr. Allen Cowley
Biophotonics lab:
Mahsa Ranji