1. Characterization of the light-responsive transdermal MNs.
Characterization of the light-responsive transdermal MNs. (A) Surface temperature changes of the MNs with or without tumor lysate in real time with continuous 808-nm NIR irradiation at 1.0 W/cm2, characterized by an infrared thermal camera (scale bars, 1 mm). (B) Quantitative surface temperature changes of representative MNs with continuous NIR irradiation at 1.0 W/cm2 (n = 3). (C) Quantitative temperature changes of representative MN patch with increasing laser power flux (n = 3). (D) In vitro collective release of tumor lysate proteins from the MN patch (n = 3). (E) In vitro activation of DCs in response to MNs loaded with tumor lysate and GM-CSF or LPS and exposed to NIR irradiation for different time (n = 3). (F) LIVE/DEAD assay of DCs after treatments with (i) blank MN, (ii) 10 min of NIR, (iii) MN, and (iv) MN and 10 min of NIR. Live cell (green), dead cell (red). (G) Quantification of DC viability after treatments. Data points represent mean ± SD (n = 3). Error bars indicate SD. Statistical significance was calculated by Student’s t test [not significant (NS), P > 0.05; *P < 0.05].
Yanqi Ye et al. Sci. Immunol. 2017;2:eaan5692
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