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T-cell cytokines in chronic allergic eye disease

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1 T-cell cytokines in chronic allergic eye disease
Daniela P. Metz, PhDa, Melanie Hingorani, FRCOphth, Virginia L. Calder, PhDa, Roger J. Buckley, FRCOphthb, Susan L. Lightman, FRCOphtha,b  Journal of Allergy and Clinical Immunology  Volume 100, Issue 6, Pages (December 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 FIG. 1 A, Upper tarsal conjunctiva in VKC demonstrates cell with immunoreactivity for both CD3 (DAB) and IL-5 (AEC) (arrow) (magnification ×40, hematoxylin counterstain). B, Negative control demonstrates no positive immunostaining (magnification ×20). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 FIG. 1 A, Upper tarsal conjunctiva in VKC demonstrates cell with immunoreactivity for both CD3 (DAB) and IL-5 (AEC) (arrow) (magnification ×40, hematoxylin counterstain). B, Negative control demonstrates no positive immunostaining (magnification ×20). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 FIG. 2 A, Multiple foci of IL-2 mRNA localization (arrowheads) in upper tarsal conjunctiva of AKC (magnification ×20, hematoxylin counterstain). B, Foci of IL-4 mRNA localization in positive control T-cell cytospin (magnification ×40, hematoxylin counterstain). C, No IL-4 mRNA localization in negative control T-cell cytospin (magnification ×40, hematoxylin counterstain). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 FIG. 2 A, Multiple foci of IL-2 mRNA localization (arrowheads) in upper tarsal conjunctiva of AKC (magnification ×20, hematoxylin counterstain). B, Foci of IL-4 mRNA localization in positive control T-cell cytospin (magnification ×40, hematoxylin counterstain). C, No IL-4 mRNA localization in negative control T-cell cytospin (magnification ×40, hematoxylin counterstain). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 FIG. 2 A, Multiple foci of IL-2 mRNA localization (arrowheads) in upper tarsal conjunctiva of AKC (magnification ×20, hematoxylin counterstain). B, Foci of IL-4 mRNA localization in positive control T-cell cytospin (magnification ×40, hematoxylin counterstain). C, No IL-4 mRNA localization in negative control T-cell cytospin (magnification ×40, hematoxylin counterstain). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

7 FIG. 3 Number of samples expressing cytokine mRNA by ISH in chronic allergic eye disease. *Significant difference versus normal controls; (*)Significant difference versus other disorders. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

8 FIG. 4 Colocalization of CD3 and cytokine by IHC in chronic allergic eye disease. A, Number of samples containing cells with colocalization of both antigens. B, Percentage of CD3 cells in which colocalization of cytokines was observed. *Significant difference versus normal controls; (*)Significant difference versus other disorders. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions


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