1. Donor and recipient BAL T cells are phenotypically and functionally memory T cells.
Donor and recipient BAL T cells are phenotypically and functionally memory T cells. (A) Subset composition of donor and recipient CD4+ and CD8+ T cells in representative patient BAL based on CD45RA and CCR7 expression as in Fig. 2. (B) Compiled data (means ± SEM) of donor (left) and recipient (right) T cell subset composition (TEM, blue; TEMRA, red; näive, green; TCM, black) over time after transplantation for CD4+ (top) and CD8+ T cell (bottom) lineages. Results compiled from 20 patients with one sample per patient per time point. (C) HLA-DR expression by donor and recipient T cells in patient BAL at indicated times after transplantation shown as representative flow cytometry plots (left) and compiled frequencies from 23 samples derived from 11 patients (right). (D to F) T cells from patient BAL samples were stimulated with PMA/ionomycin, and cytokine production was determined by intracellular staining after 5 hours. (D) IFN-γ production by donor (black) and recipient (red) CD4+ TEM (left) and CD8+ TEM (right) in representative flow cytometry plots and graphs showing paired frequencies (based on percent cytokine+ of donor or recipient T cells) in patient BAL samples (n = 8) 1 to 9 months after transplantation (right). *P = 0.04 for CD4+ TEM and *P = 0.01 for CD8+ TEM. (E) IL-17 production by donor (black) and recipient (red) CD4+ TEM in representative flow cytometry plot (left) and graph (right) showing paired frequencies in patient BAL samples (n = 7) 1 to 9 months after transplantation. *P = (F) IL-2 production by donor (black) and recipient (red) CD4+ TEM in representative flow cytometry plot (left) and graph showing paired frequencies in patient BAL samples (right; n = 8). (G) Granzyme B (GzB) expression by CD4+ TEM (left) and CD8+ TEM (right) from BAL samples obtained >2 months after transplantation in representative flow cytometry plots (left) and cumulative paired data (right) from six transplant recipients of donor (black) and recipient (red). *P = 0.04.
Mark E. Snyder et al. Sci. Immunol. 2019;4:eaav5581
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