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Renal citrate metabolism and urinary citrate excretion in the infant rat
Joel Z. Melnick, Patricia A. Preisig, Robert J. Alpern, Michel Baum Kidney International Volume 57, Issue 3, Pages (March 2000) DOI: /j x Copyright © 2000 International Society of Nephrology Terms and Conditions
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Figure 1 Sodium (Na)/citrate cotransporter activity was determined in adult (□) and infant (■) rats. Na-dependent (left bars) and Na-independent (right bars) 14C-citrate uptake was determined in renal cortical brush border membrane vesicles (N = 6). Kidney International , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions
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Figure 2 Citrate synthase activity in adult and infant rats. Citrate synthase activity was determined spectrophotometrically as the appearance of CoA from acetyl CoA and oxaloacetate in mitochondrial suspensions (N = 6). Kidney International , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions
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Figure 3 Adenosine 5′-triphosphate (ATP) citrate lyase activity in adult and infant rats. ATP citrate lyase activity was measured spectrophotometrically as the generation of acetylhydroxamate (N = 6). *P < 0.05. Kidney International , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions
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Figure 4 Mitochondrial-aconitase (m-aconitase) activity in adult and infant rats. m-Aconitase activity was determined spectrophotometrically as aconitate generation from isocitrate in mitochondrial suspensions (N = 6). *P < 0.05. Kidney International , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions
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Figure 5 Renal cortical citrate concentration in adult and infant rats. Kidneys were freeze clamped in vivo, and pulverized under liquid N2. Citrate levels were measured spectrophotometrically (N = 9). Kidney International , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions
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