1. Msp1 is a conserved mitochondrial outer membrane protein Protein sequence alignment and domain prediction of yeast, fly, mouse, and human homologs (SDSC Biology Workbench).
Msp1 is a conserved mitochondrial outer membrane protein Protein sequence alignment and domain prediction of yeast, fly, mouse, and human homologs (SDSC Biology Workbench). The transmembrane domain (TMD) is marked by a dashed cyan box (dashed because the endpoints of the TMD are not clearly defined), and the AAA+ domain is marked by a yellow box. Glutamate 193 is indicated by an asterisk (*). Representative images of the msp1Δ mutant expressing Msp1‐GFP and Mito‐RFP. Yeast were grown to early log phase at 30°C in synthetic glucose (SD) medium and visualized by fluorescence microscopy. Peroxisomal Msp1‐GFP signal is marked by arrows. Intact, hypotonically swollen and Triton X‐100‐solubilized mitochondria of the msp1Δ mutant expressing Msp1‐His6/HA3 were treated with (+) or without (−) Proteinase K and analyzed by immunoblot with whole‐cell lysate (WCL) and post‐mitochondrial supernatant (PMS). Mge1, Cyb2, and Fzo1 are matrix, intermembrane space (IMS), and outer membrane proteins (OMM), respectively. Soluble (S) and pellet fraction (P) of the KCl‐ or Na2CO3‐treated mitochondria purified from the strain described in (C) were analyzed by immunoblot. Cyb2 is a peripherally attached mitochondrial inner membrane protein; Por1 and Om45 are both integral outer membrane proteins. The asterisk (*) indicates a non‐specific band. Human dermal fibroblasts (HDFs) stably expressing ATAD1‐GFP were stained with Mitotracker Red and visualized by florescence microscopy. Source data are available online for this figure.
Yu‐Chan Chen et al. EMBO J. 2014;33:
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