1. Aggregation is not required for cytoplasmic relocalization induced by misfolding mutations.
Aggregation is not required for cytoplasmic relocalization induced by misfolding mutations. (A) Schematic representation of the Nter-NLS-Cter construction (see Fig. 4A) with positions of the misfolding mutations. (B) Images from fluorescence microscopy acquisitions showing the delocalization induced by misfolding mutations. See Fig. 1 for details. Scale bars: 2 μm. (C) Quantification of B (see Table S1 for details, including sample sizes). Bars and whiskers correspond, respectively, to the median and extreme values of three independent transformants. The star indicates a significant difference in median proportions compared to WT cells (Fisher exact test). 23% of the Nter-NLS-Cter-mCherry cells showing a diffusive signal in the nucleus presented an additional tiny fluorescent spot in the cytoplasm. With W1837C and Y1853X, tiny fluorescent spots were visualized in respectively 44% and 35% of the cells showing a diffusive signal in both the cytoplasm and nucleus. The slight differences with Fig. 4C result from a better sensitivity used during picture acquisitions because of the absence of intense fluorescent spots. Cyto+Nuc, cytoplasm and nuclear localization; Nuc, nuclear localization.
Pierre Thouvenot et al. J Cell Sci 2016;129:
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