1. ATPase activity of the ABCE1 disengagement and mixed mutants.
ATPase activity of the ABCE1 disengagement and mixed mutants. (A) Representative set of three independent TLC radiograms of 32P-γ-ATP (5 mM) and analysis of the ATPase assay for the SR variants of ABCE1 (1 μM). ATP turnover of all ABCE1 variants was determined as the initial rate constants of the exponential increase of inorganic phosphate in a time window of 20 min. For the hyperactive ABCE1E485A, ATP turnover was determined within the first 5 min. (B) Radiograms (n = 3) and analysis of the ATPase assay for mutants with mixed mutations; disengagement in site I and catalytic base exchange in site II or vice versa. (C) Overview of the ATPase activity for all mutants in this study at optimal conditions (70°C, 2.5 mM Mg2+).
Elina Nürenberg-Goloub et al. LSA 2018;1:e
© 2018 Nürenberg-Goloub et al.