1. Coomassie
αLA
LipS in vitro activity. Recombinant LipS was purified and assayed in the presence of protein substrate and cofactor S-adenosylmethionine (SAM). The product of SAM cleavage (5’ dA) was monitored over the course of LipS reaction.
E1 αKGDH
E2 PDHC
LipS
E3 αKGDH
and PDHC
E2 αKGDH
H Protein
Malaria LipS binds to the 3 lipoate-dependent enzyme complexes found in E. coli. The labeled bands were identified by amino acid sequencing and/or anti-lipoate Western blot. Tight LipS/substrate complexes will now be used to stabilize LipS, and to characterize the mechanism guiding substrate specificity.