1. Gene expression is reduced when RNAP binds a promoter too tightly.
Gene expression is reduced when RNAP binds a promoter too tightly. Shown is measured expression vs. inferred promoter strength −ΔERNAP (stronger promoters on the right). Expression decreases once the RNAP binding becomes comparable to the free energy of the transcription initiation state −ΔEtrans=6.2 kBT. The dashed line shows the prediction of the multivalent model.
Tal Einav, and Rob Phillips PNAS doi: /pnas
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