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Fig. 1. Representative images of the four cell lines using fluorescence microscopy.
Representative images of the four cell lines using fluorescence microscopy. Each set of two panels represent the low metastatic (left) and the high metastatic (right) partner of a paired cell line. The cells nuclei (blue), the actin cytoskeleton (green) and the lipid membrane (red) of fixed cells are stained and are pseudo-colored as indicated for contrast. Note that the yellow color indicates the overlap of the red (membrane) and green (actin) channels. The cell lines are: (A) Dunn, (B) DLM8, (C) K12, (D) K7M2, (E) Saos2, (F) SAOS-LM7, (G) MG63 and (H) MG63.2. In all panels, scale bar is 50 μm. Samanthe M. Lyons et al. Biology Open 2016;bio © Published by The Company of Biologists Ltd
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