1. Volume 71, Issue 4, Pages 325-335 (February 2007)
Gene expression profiles of human proximal tubular epithelial cells in proteinuric nephropathies 
M. Rudnicki, S. Eder, P. Perco, J. Enrich, K. Scheiber, C. Koppelstätter, G. Schratzberger, B. Mayer, R. Oberbauer, T.W. Meyer, G. Mayer 
Kidney International 
Volume 71, Issue 4, Pages (February 2007)
DOI: /sj.ki
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2. Figure 1
Taqman real-time PCR analysis of five selected genes from Table 1. DEFB1 (Hs.32949), TIMP1 (Hs ), DUSP5 (Hs.2128), BMP7 (Hs ) and RAB4A (member of the ras oncogene family, Hs ). All numbers represent log-transformed fold-change values between patients and controls where positive values indicate higher expression in patients. The real-time PCR results were calculated as relative expression to the internal standard glyceraldehyde-3-phosphate dehydrogenase using the delta–delta-ct method. Real-time experiments showed a Pearson's correlation of 0.81 when compared to Microarray results of the seven selected patients, and 0.79 when compared to expression values from all patients, respectively (see Materials and Methods section).
Kidney International  , DOI: ( /sj.ki )
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3. Figure 2
Immunohistochemical staining of TSP-3. (a, b) Immunostaining for TSP-3: original magnification × 40. (a) In control subjects we found a weak TSP-3 signal in all tubule cells. (b) In proteinuric patients a strong positive staining for TSP-3 was observed in most of the tubule cells.
Kidney International  , DOI: ( /sj.ki )
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4. Figure 3
Immunohistochemical staining of BMP-7. (a, b) Immunostaining for BMP-7: original magnification × 20. (a) In healthy subjects we did not detect any positive BMP-7 signal in proximal tubules and neither in the glomeruli. Arrows: there are single distal tubules with a weak BMP-7 staining. (b) Stable proteinuric patients showed a strong BMP-7 staining in tubule cells. Interestingly, we found the most prominent staining at the luminal side of the cells.
Kidney International  , DOI: ( /sj.ki )
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5. Figure 4
Staining for alkaline phosphatase to identify proximal tubule cells. Original magnification × 40: after staining for alkaline phosphatase using 4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP), the proximal tubule cells were identified by showing a strong dark blue staining.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2007 International Society of Nephrology Terms and Conditions