1. Discovery of germ cell–specific transcripts by expressed sequence tag database analysis 
Aleksandar Rajkovic, M.D., Ph.D., Changning Yan M.S, Michal Klysik, Martin Matzuk, M.D., Ph.D. 
Fertility and Sterility 
Volume 76, Issue 3, Pages (September 2001)
DOI: /S (01)

2. Figure 1
RT-PCR analysis of germ cell–specific transcripts. First strand cDNA from heart, liver, lung, brain, spleen, kidney, uterus, testis, intestine, and ovary tissues was amplified with oligonucleotides specific for the particular EST.
Rajkovic. Germ cell–specific transcripts. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

3. Figure 2
Tissue–specific expression of 2077 transcripts. Fifteen micrograms of total RNA was loaded in each lane and hybridized to either 2077 or 18S rRNA cDNA labeled with [α-32P]dATP. Arrowheads indicate the location of 2077 and 18S rRNA transcripts. The position of migration of the 2077 mRNA relative to 18S rRNA and 28S rRNA is indicated in the upper panel. Three possible transcripts can be discerned in RNA isolated from testis.
Rajkovic. Germ cell–specific transcripts. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )

4. Figure 3
In situ hybridization on 12-week-old wild-type mouse ovaries using antisense riboprobe generated from 511, 2077, and Panels A, C, and E show brightfield view of H&E stained mouse ovaries. Panels B, D, F are darkfield views showing that 511, 2077, and 7719 are expressed exclusively in the oocytes.
Rajkovic. Germ cell–specific transcripts. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01) )