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Representative flow cytometric analysis of bone marrow, spleen and whole blood from EpoWT/WT, EpoΔ/Δ and EpoWT/Δ mice. Representative flow cytometric analysis.

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Presentation on theme: "Representative flow cytometric analysis of bone marrow, spleen and whole blood from EpoWT/WT, EpoΔ/Δ and EpoWT/Δ mice. Representative flow cytometric analysis."— Presentation transcript:

1 Representative flow cytometric analysis of bone marrow, spleen and whole blood from EpoWT/WT, EpoΔ/Δ and EpoWT/Δ mice. Representative flow cytometric analysis of bone marrow, spleen and whole blood fromEpoWT/WT,EpoΔ/ΔandEpoWT/Δmice. Tissue was collected, processed through a 40-μm strainer, and stained for anti-CD71 and -Ter119 antibodies for 45 minutes. nexperiments=4. Whole blood was diluted and stained for thiazole orange for 30 minutes, then analyzed on BD FACS Caliber. nexperiments=4. Brandon M. Zeigler et al. Dis. Model. Mech. 2010;3: ©2010 by The Company of Biologists Limited

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10 0 1 2 3 4 0 1 2 3 4 59% Ter119 CD71 10 0 1 2 3 4 0 1 2 3 4 13% 10 0 1 2 3 4 0 1 2 3 4 1.5% +/+ki/ki 10 0 1 2 3 4 0 20 40 60 80 100 2.71 10 0 1 2 3 4.

% Ter119 CD % % +/+ki/ki
S2 Fig. Phenotype of donor cells in the spleen and bone marrow of male mice. Splenocytes and bone marrow were isolated from male donor mice and stained.

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