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Fig. 4. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. Expression of oxt is undetectable in.

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Presentation on theme: "Fig. 4. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. Expression of oxt is undetectable in."— Presentation transcript:

1 Fig. 4. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. A-C are all dorsal views of WISH for oxt in 48 hpf embryos magnified ×100 in which arrowheads indicate oxt expression. Embryos treated with IWR and med12 mutants were compared to sibling controls. (A) wild-type embryo. (B) med12 mutant embryo. (C) IWR-treated embryo. (D) oxt expression was measured using RT-qPCR in 48 hpf embryos. Gene expression values were normalized to an endogenous control, beta actin 2 (actb2), and are expressed as fold changes relative to wild-type sibling, or vehicle-treated wild-type sibling controls. A: P<0.01, two-tailed paired Student's t-test. Dot plot shows three biological replicates (black) and the mean (red). (E,E′,F,F′) Representative images of GFP expression was visualized in 24 hpf vehicle and IWR-treated embryos that are heterozygous for the TOP:GFP transgene. (E,E′) Magnified ×100. Arrows indicate area of reduced GFP expression. (F,F′) Representative full embryo views magnified ×50. Emma D. Spikol, and Eric Glasgow Biology Open 2018;7:bio031229 © Published by The Company of Biologists Ltd

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