1. Targeting of endothelial Rbpj during vessel remodeling.
Targeting of endothelial Rbpj during vessel remodeling. (A) Timeline of the experimental procedure. (B) Confocal images of whole-mount P28 Pdgfb-iCre × Rbpjflox/flox (RbpjiΔEC) mutants and control littermates treated with tamoxifen from P10 onwards (P10+). Note the altered morphology of RbpjiΔEC distal vein and perivenous plexus. Boxed regions are shown at higher magnification in C. a, artery; v, vein. (C) Immunostaining showing junctional VE-cadherin (red) and nuclear Erg (green) together with Isolectin B4 in P28 RbpjiΔEC and control retinas. Mutant veins were enlarged and contained more Erg+ ECs. RbpjiΔEC perivenous capillaries showed high-density and numerous thin protrusions. (D) Analysis of vein caliber, venous EC number and size. P-values by Student’s t-test. Error bars indicate s.e.m. (E) Visualization of empty matrix sleeves by Isolectin B4 (green) and collagen IV (red) staining. Sprouts and filopodia are indicated by arrowheads, empty matrix sleeves by arrows.
Manuel Ehling et al. Development 2013;140:
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