1. Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss.
In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. (A) Experimental design. Mouse fetuses at E14.5 were injected in utero with cytokines or T cells, and the survival rate was quantified at birth. (B) Representative graphs of the percentage of delivered pups after fetal injection with TNF-α or IFN-γ. (C) Representative graphs of the percentage of delivered pups after fetal adoptive transfer of T cells. CD4+ and CD8+ T cells from C57BL/6, IFN-γ−/− or TNF-α−/− mice were activated in vitro for 3 days with anti-CD3 and anti-CD28 antibodies before fetal injection. Nonactivated T cells from C57BL/6 mice were used as control (n ≥ 4 experiments, with n ≥ 3 dams per group). Small horizontal bars indicate the mean ± SEM. ***P < by one-way ANOVA with Bonferroni correction.
Michela Frascoli et al., Sci Transl Med 2018;10:eaan2263
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