1. Native MS characterization of KaiCAA nucleotide state depending on external ATP/ADP condition.
Native MS characterization of KaiCAA nucleotide state depending on external ATP/ADP condition. (A–F) Native mass spectra of KaiCAA mediated by (A–C) ATP and (D–F) AMPPNP. The KaiCAA hexamers incubated for 5 h at 37°C under (A, D) 100:0, (B, E) 75:25, and (C, F) 50:50 ATP/ADP conditions were immediately analyzed by nanoflow electrospray ionization MS. The blue and purple circles show the ion series of the KaiCAA hexamers containing seven ATP/five ADP molecules and 12 AMPPNP molecules, respectively.
Yasuhiro Yunoki et al. LSA 2019;2:e
© 2019 Yunoki et al.