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Fig. 6. Hts regulates par-1 and camkII mRNA distribution and levels in the muscle.(A–J) Views of muscles 6/7 in abdominal segment 4, probed for either par-1 or camkII mRNA (red). Hts regulates par-1 and camkII mRNA distribution and levels in the muscle.(A–J) Views of muscles 6/7 in abdominal segment 4, probed for either par-1 or camkII mRNA (red). Nuclei are marked with DAPI (blue). (A,F) In wild-type larval body walls, par-1 and camkII mRNA are predominately sequestered in muscle nuclei. (B,C,G,H) In contrast, expression of either wild-type or non-phosphorylatable Hts with mef2-Gal4 causes par-1 and camkII mRNA, observed as distinct puncta, to be dispersed throughout the muscle cytoplasm. (D,I) Expression of phospho-mimetic Hts, on the other hand, results in overall reductions in par-1 and camkII mRNA levels in both the muscle nuclei and cytoplasm. (E,J) hts01103 mutant larval body walls also show reduced par-1 and camkII mRNA levels. Scale bar in Panel J represents 20 µm (for A–J). Simon Ji Hau Wang et al. Biology Open 2014;bio © Published by The Company of Biologists Ltd
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