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Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

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1 Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle
Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle.(A) Expression of abu-5 (red) and abu-11 (green) in pharyngeal cells. abu/pqn genes are expressed in the pharyngeal cuticle.(A) Expression of abu-5 (red) and abu-11 (green) in pharyngeal cells. Scale bar is 10 µm. (B) Differential interference contrast (DIC) and fluorescence image of the anterior pharynx of an adult transgenic animal containing the transgene abu-14>abu-14::sfGFP. The fluorescence localizes to the pharyngeal corpus and buccal cavity cuticle (arrows). A non-transgenic sister (marked with “non-Tg”) shows no fluorescence. Anterior is to the left. Scale bar is 10 µm. (C) DIC and fluorescence image of the pharynx of a fourth larval stage molting animal containing the transgene abu-14>abu-14::sfGFP. Green fluorescence is seen in both the L4 and adult pharyngeal grinders, in the pharyngeal cuticle, and in the buccal cap. Anterior is to the left. Scale bar is 10 µm. (D) DIC (left) and fluorescence (right) images of a larval animal containing the transgene abu-7>abu-7::GFP. The pharyngeal grinder is brightly fluorescent. Scale bar is 10 µm. Julia B. George-Raizen et al. Biology Open 2014;bio © Published by The Company of Biologists Ltd

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