Presentation is loading. Please wait.

Presentation is loading. Please wait.

Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced.

Published byPaula Poblete Modified over 5 years ago

Similar presentations

Presentation on theme: "Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced."— Presentation transcript:

1 Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced airway obstruction  Siobhán O’Sullivan, BSca,b, Barbro Dahlén, MD, PhDc, Sven-Erik Dahlén, MD, PhDa,d, Maria Kumlin, PhDb,d  Journal of Allergy and Clinical Immunology  Volume 98, Issue 2, Pages (August 1996) DOI: /S (96) Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 Accuracy of EIA. Known amounts of synthetic 9α,11β-PGF2 added to buffer (open squares) and unextracted urine (filled triangles) were measured with EIA. Accuracy was excellent, with r2 = 0.99 in both buffer and urine (mean ± SEM, n = 8). Conc, Concentration. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 2 Chromatographic validation of immunoreactivity. A, RP-HPLC of synthetic standard 9α,11β-PGF2 in buffer, after purification by solid-phase extraction (for details see Methods). The compound was detected as EIA immunoreactivity (filled circles) and UV absorbance (unbroken line). B, RP-HPLC of a purified urine sample collected from a patient with atopic asthma after allergen challenge. Filled circles show 9α,11β-PGF2 immunoreactivity in the fractions assessed by EIA. Losses of 9α,11β-PGF2 during purification were corrected for by use of tritiated PGF2α as internal standard. Open squares show distribution of tritiated PGF2α as determined by liquid scintillation counting. The amount of endogenous urinary 9α,11β-PGF2 was below the detection limit for UV absorbance measurements. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 2 Chromatographic validation of immunoreactivity. A, RP-HPLC of synthetic standard 9α,11β-PGF2 in buffer, after purification by solid-phase extraction (for details see Methods). The compound was detected as EIA immunoreactivity (filled circles) and UV absorbance (unbroken line). B, RP-HPLC of a purified urine sample collected from a patient with atopic asthma after allergen challenge. Filled circles show 9α,11β-PGF2 immunoreactivity in the fractions assessed by EIA. Losses of 9α,11β-PGF2 during purification were corrected for by use of tritiated PGF2α as internal standard. Open squares show distribution of tritiated PGF2α as determined by liquid scintillation counting. The amount of endogenous urinary 9α,11β-PGF2 was below the detection limit for UV absorbance measurements. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

5 FIG. 3 EIA analysis of 9α,11β-PGF2 (filled circles) in unpurified urine samples collected at three hourly intervals over a 24-hour period, from healthy nonasthmatic subjects (mean ± SEM, n = 5). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

6 FIG. 4 Allergen-induced urinary excretion of 9α,11β-PGF2. Mean hourly levels of 9α,11β-PGF2 (filled squares) assayed in urine samples of nine patients with atopic asthma, undergoing bronchial provocation with allergen. Arrow indicates the time of maximum drop in FEV1 (mean ± SEM, n = 9). The difference in pre- and postchallenge levels of 9α,11β-PGF2 was statistically significant (p < 0.05). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

7 FIG. 5 Aspirin-induced urinary excretion of 9α,11β-PGF2. Prechallenge (open bars) and postchallenge (hatched bars) levels of 9α,11β-PGF2 in urine samples from eight patients with aspirin-intolerant asthma after challenge with lysine aspirin. A, Drug naive control session (placebo). B, After pretreatment with the LT-receptor antagonist MK-0679 (drug session). All patients received higher doses of lysine aspirin during the MK-0679 session (B). The urine sample collected immediately before inhalation challenge with lysine aspirin is defined as the prechallenge level, and the peak detected after maximal airway obstruction, as the postchallenge level (mean ± SEM). Levels of statistical significance are indicated by asterisks (Student’s t test, *p < 0.05, **p < 0.01). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

8 FIG. 5 Aspirin-induced urinary excretion of 9α,11β-PGF2. Prechallenge (open bars) and postchallenge (hatched bars) levels of 9α,11β-PGF2 in urine samples from eight patients with aspirin-intolerant asthma after challenge with lysine aspirin. A, Drug naive control session (placebo). B, After pretreatment with the LT-receptor antagonist MK-0679 (drug session). All patients received higher doses of lysine aspirin during the MK-0679 session (B). The urine sample collected immediately before inhalation challenge with lysine aspirin is defined as the prechallenge level, and the peak detected after maximal airway obstruction, as the postchallenge level (mean ± SEM). Levels of statistical significance are indicated by asterisks (Student’s t test, *p < 0.05, **p < 0.01). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

9 FIG. 6 No increase in urinary 9α,11β-PGF2 after bronchoprovocation with histamine. Prechallenge (open bar) and postchallenge (filled bar) levels of 9α,11β-PGF2 after duplicate histamine provocations in four aspirin-intolerant patients with asthma. (The result for each individual is the mean of two different provocations, done at least 6 weeks apart). Values are expressed as means ± SEM. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

Download ppt "Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced."

Similar presentations

Volume 100, Issue 2, Pages (February 2006)

Volume 100, Issue 2, Pages (February 2006)
Effect of 2-year placebo-controlled immunotherapy on airway symptoms and medication in patients with birch pollen allergy  Monica B. Arvidsson, MD, Olle.

Effect of 2-year placebo-controlled immunotherapy on airway symptoms and medication in patients with birch pollen allergy  Monica B. Arvidsson, MD, Olle.
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.
The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,
Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance  Ulf Bengtsson, MD, PhDa, Tina W. Knutson,

Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance  Ulf Bengtsson, MD, PhDa, Tina W. Knutson,
Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.
Protective effect of inhaled budesonide against unlimited airway narrowing to methacholine in atopic patients with asthma  Patrick Booms, MSca, David.

Protective effect of inhaled budesonide against unlimited airway narrowing to methacholine in atopic patients with asthma  Patrick Booms, MSca, David.
Ulf Darsow, MD, Dieter Vieluf, MD, Johannes Ring, MD, PhD 

Ulf Darsow, MD, Dieter Vieluf, MD, Johannes Ring, MD, PhD 
The Editors' Choice Journal of Allergy and Clinical Immunology

The Editors' Choice Journal of Allergy and Clinical Immunology
Inhibitory effect of a leukotriene receptor antagonist (montelukast) on neurokinin a- induced bronchoconstriction  Nunzio Crimi, MD, Corrado Pagano, MD,

Inhibitory effect of a leukotriene receptor antagonist (montelukast) on neurokinin a- induced bronchoconstriction  Nunzio Crimi, MD, Corrado Pagano, MD,
Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine  Amato de Paulis,

Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine  Amato de Paulis,
Aspirin-induced urticaria and angioedema, but not bronchoconstriction, associated with cysteinyl leukotriene overproduction in 2 patients with asthma 

Aspirin-induced urticaria and angioedema, but not bronchoconstriction, associated with cysteinyl leukotriene overproduction in 2 patients with asthma 
Dose-dependent effects of capsaicin nasal challenge: In vivo evidence of human airway neurogenic inflammation  Alvin M. Sanico, MDa, Satsuki Atsuta, MDa,

Dose-dependent effects of capsaicin nasal challenge: In vivo evidence of human airway neurogenic inflammation  Alvin M. Sanico, MDa, Satsuki Atsuta, MDa,
Deficient prostaglandin E2 production by bronchial fibroblasts of asthmatic patients, with special reference to aspirin-induced asthma  Malgorzata Pierzchalska,

Deficient prostaglandin E2 production by bronchial fibroblasts of asthmatic patients, with special reference to aspirin-induced asthma  Malgorzata Pierzchalska,
T lymphocytes in asthma: Bronchial versus peripheral responses

T lymphocytes in asthma: Bronchial versus peripheral responses
Badrul A. Chowdhury, MD, PhD 

Badrul A. Chowdhury, MD, PhD 
Comparison of the effects of terfenadine with fexofenadine on nasal provocation tests with allergen  Maria-Helena Terrien, MD, François Rahm, MD, Jean-Marc.

Comparison of the effects of terfenadine with fexofenadine on nasal provocation tests with allergen  Maria-Helena Terrien, MD, François Rahm, MD, Jean-Marc.
Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.
Rhinitis and asthma: Evidence for respiratory system integration

Rhinitis and asthma: Evidence for respiratory system integration

Similar presentations

Ads by Google