1. Gel Electrophoresis Analysis
Using a DNA “ladder” composed of fragments of known length, you can determine the size of unknown DNA fragments

2. Typical Gel with HindIII Cut l Marker
Measure Marker Fragments
1.8 cm
3.0 cm
3.6 cm
4.8 cm
7.8 cm
8.4 cm
Measure Unknown Fragment of Interest
Unknown Fragment
5.9cm

3. 100 10 1
Fragment Length (Kb)
3.2 Kb
Fragment Migration Distance (cm)
5.9 cm

4. Using Excel Plot data in Excel semi-log chart
Fit a power curve and display the equation for the fit
Substitute unknown migration distance for X and solve