1. Import of recombinant Xp54 into the oocyte nucleus and binding to nascent RNP is dependent on the transcriptional state of the chromatin.
Import of recombinant Xp54 into the oocyte nucleus and binding to nascent RNP is dependent on the transcriptional state of the chromatin. (A) The nucleocytoplasmic distribution of T7-p54, T7-ΔV and T7-ΔCT expressed in stage VI oocytes compared with binding of the recombinant proteins to the RNP matrix of lampbrush chromosomes is shown. The chromosome spreads were immunostained with anti-T7 IgG and FITC-conjugated anti-mouse IgG, post-stained with DAPI. Phase contrast (PC) microscopy images are also shown. (B) As described in (A) but with treatment of the oocytes with 2 ng/ml trichostatin A (TSA) or 0.5 μg/ml α-amanitin (α-AM) for two hours before addition of LMB. (C) Sedimentation of T7-p54 in complexes isolated from 50 stage VI oocytes and from nuclei and cytoplasms of 50 stage VI oocytes treated with TSA.
David A. Smillie, and John Sommerville J Cell Sci 2002;115:
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