1. Distribution and levels of phosphorylated PKC in L
Distribution and levels of phosphorylated PKC in L. stagnalis haemocytes investigated by immunocytochemistry.
Distribution and levels of phosphorylated PKC in L. stagnalis haemocytes investigated by immunocytochemistry. Phosphorylated PKC in (A) untreated haemocytes, (B) haemocytes challenged with laminarin (10 mg ml-1) for 10 min, and (C) haemocytes incubated with GF109203X (10μ mol l-1) for 30 min prior challenge with laminarin (10 mg ml-1) for 10 min. Rhodamine phalloidin (red) stains F-actin and fluorescein (green) shows the phosphorylated PKC detected with the anti-phospho PKC (pan) antibody. Haemocytes were observed with a Leica laser scanning confocal microscope. Results are representative of three independent experiments. Bar, 20 μm.
Audrey H. Lacchini et al. J Exp Biol 2006;209:
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