1. Susceptibility of rat AECII in primary culture to IAV infection is reduced by rapamycin (mTOR inhibitor) or BGJ398 (FGFR inhibitor).
Susceptibility of rat AECII in primary culture to IAV infection is reduced by rapamycin (mTOR inhibitor) or BGJ398 (FGFR inhibitor). AECII were isolated from Sprague-Dawley rats and grown on collagen-IV coated tissue culture plates. (A) Light micrograph of typical morphology of AECII cells after 4 d of incubation in culture. (B) Lysotracker-labeled monolayers demonstrating prominent lamellar bodies associated with AECII. (C) Western blot of KGF (50 ng/mL) induced increases in PI3K/mTOR signaling in rat AECII. (D) Western blot of AECII cell lysates 18 h after IAV infection is conducted in the presence or absence of KGF (50 ng/mL) in cells and pretreatment with FGFR pan-inhibitor 2.5 µM BGJ398 or the mTOR inhibitor 40 nM rapamycin. Blots were stained with antibodies to IAV M1 and to actin as a loading control.
Nikolaos M. Nikolaidis et al. PNAS 2017;114:32:E6613-E6622
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