1. No correlation between the position of the gene in the nuclear volume and the transcription levels on each of the alleles.
No correlation between the position of the gene in the nuclear volume and the transcription levels on each of the alleles. (A) Example of 3D rendering of the nuclear volume based on Hoechst staining. (B) Plot showing the frequency of active genes in relation to their distance from the center of the nucleus (duplicated sites after TSA). (C) Distribution plot comparing the number of nascent transcripts quantified on each alleles versus the distance from the center of the nucleus. Number of cells quantified (n) is marked in red. (D) Frames from time-lapse imaging showing the intensity of MS2-CP-GFP on the active duplicated and separated genes over time. Arrows point to the active genes. Scale bar, 5 μm.
Sharon Yunger et al. LSA 2018;1:e
© 2018 Yunger et al.