1. Smo deficiency in the renal stroma is dependent on GLI3R.
Smo deficiency in the renal stroma is dependent on GLI3R. (A-H) Hematoxylin and Eosin-stained images reveal that deletion of Gli3 rescues nephrogenic zone patterning and capsular defects seen in Smo-deficient mutants (A-C,E-G), whereas introduction of GLI3R phenocopies the Smo-deficient mutants (D,H). Arrowheads indicate the renal capsule (E-H). (I-L) Immunostaining for SIX2 shows that deletion of Gli3 rescues the expanded condensing mesenchyme (K) and obligate stromal GLI3R causes an expansion in condensing mesenchyme (L, arrowheads). (M,N) Removal of Gli3 partially, but not completely, rescues kidney volume (M; 10% decrease, control versus mutant, n=6, **P=0.0081) and nephron number (N; 15% reduction, control versus mutant, n=6, ***P<0.001) at E18.5. Data are mean±s.e.m. Scale bars: 1000 µm in A-D; 20 µm in E-H; 100 µm in I-L.
Christopher J. Rowan et al. Development 2018;145:dev159947
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