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Xiangfeng Cui, Helen Feiner, Honghua Li 

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Presentation on theme: "Xiangfeng Cui, Helen Feiner, Honghua Li "— Presentation transcript:

1 Multiplex Loss of Heterozygosity Analysis by Using Single or Very Few Cells 
Xiangfeng Cui, Helen Feiner, Honghua Li  The Journal of Molecular Diagnostics  Volume 4, Issue 3, Pages (August 2002) DOI: /S (10)60699-X Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Schematic presentation of the multiplex PCR protocol. Three loci are shown. The Journal of Molecular Diagnostics 2002 4, DOI: ( /S (10)60699-X) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Part of results from LOH analysis with single or very few cells. M: MspI digest of pBR322 DNA as molecular markers. As shown, the “+” allele of locus 2 is missing in lanes 3 and 9 (lanes are counted from left to right), and the “−” allele is missing in lane 12. The “−” allele of locus 10 is missing in lane 6. The Journal of Molecular Diagnostics 2002 4, DOI: ( /S (10)60699-X) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Part of the results from LOH analysis with 30 breast cancer cells. Eight samples with either complete LOH or allelic reduction for the GLANS (−) allele are shown on the left and results from genotyping with normal components from the same tissue specimen are shown on the right. A non-specific band that does not affect detection is indicated by the arrow. The Journal of Molecular Diagnostics 2002 4, DOI: ( /S (10)60699-X) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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