1. Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate.
Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. (A) Experimental design and timeline. CK5-CreERT2 or CK8-CreERT2; Nkx3.1+/+ or Nkx3.1−/−; YFP mice were induced with tamoxifen at 2 months of age and inoculated with a single dose of CP1 (or PBS as a control) 1 month after tamoxifen induction. Expression of specific markers was analyzed and quantified 3 months post-inoculation. Whole-mount images are provided in Fig. S5. (B) Quantification of YFP-positive cells that co-express CK5 (red), CK8 (gray) or both CK5 and CK8 (black) using the CK5-CreERT2 (left) or the CK8-CreERT2 (right) driver. For each group, at least three animals were analyzed (n numbers in Table S2), and the quantification of the lineage-marked cells is provided in Table 1. A two-tailed Welch's t-test was performed to compare the percentage of YFP-positive cells that co-express Ck5 or Ck8 in all groups. Significant differences are indicated (***P<0.001). Data are represented as means and the error bars represent standard error (s.e.m.) for each group. (C,D) Lineage tracing with CK5-CreERT2 (C) and CK8-CreERT2 (D). Representative images of immunofluorescence staining with the indicated antibodies. Insets show higher magnification of a representative region. Scale bars: 25 µm.
Clémentine Le Magnen et al. Dis. Model. Mech. 2018;11:dmm035139
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