1. DNA
Crash course….

3. A K J I B H E G D C F Amylase Lipase Pepsin Mucus Collagen Insulin
Keratin
Melanin D C
Antibodies
Muscle F
Haemoglobin

5. A T C G
x 3,000,0000,000… (in human genome)

6. ATCGGGCCATTCGAATCTAGCATTGAAACGAGTGAGCGAAACGAGCGACTTATTTGGCTAGACAGCTGAGCAGTCGACTCCAGCTGACAGCTGACGACTGAGAAGAGGCTCCAGCTCAGACAGCTAGCGACCTTCAGCTAGCTAGCGGCAGTCAGCTTCAGCTGAACGCAGCTAGCCCTTCAGGACTCTCAGGAGCTCCTCGACAGAGCAGCGATCAGCAGCTTAGGAGAGAGCCTTCAGGAGTCAGGTTTCCATCGGTCGGTACGGTACGACAGCTGAGAGCTCTAGCTAGCCCTTCAGCAGCTAGCTGACTAGGACGACGACTAGCGTACGTACTGACTTAGAGCGACTCTCTCCTAGGACTAGCTAGCTAGGAGCTCTCTCAGACGCTAGAGCTGACCTCTCTCAGAGAGCTAGCTAGCGCGAGTATCGGGCCATTCGAATCTAGCATTGAAACGAGTGAGCGAAACGAGCGACTTATTTGGCTAGACAGCTGAGCAGTCGACTCCAGCTGACAGCTGACGACTGAGAAGAGGCTCCAGCTCAGACAGCTAGCGACCTTCAGCTAGCTAGCGGCAGTCAGCTTCAGCTGAACGCAGCTAGCCCTTCAGGACTCTCAGGAGCTCCTCGACAGAGCAGCGATCAGCAGCTTAGGAGAGAGCCTTCAGGAGTCAGGTTTCCATCGGTCGGTACGGTACGACAGCTGAGAGCTCTAGCTAGCCCTTCAGCAGCTAGCTGACTAGGACGACGACTAGCGTACGTACTGACTTAGAGCGACTCTCTCCTAGGACTAGCTAGCTAGGAGCTCTCTCAGACGCTAGAGCTGACCTCTCTCAGAGAGCTAGCTAGCGCGAGT
ATCGGGCCATTCGAATCTAGCATTGAAACGAGTGAGCGAAACGAGCGACTTATTTGGCTAGACAGCTGAGCAGTCGACTCCAGCTGACAGCTGACGACTGAGAAGAGGCTCCAGCTCAGACAGCTAGCGACCTTCAGCTAGCTAGCGGCAGTCAGCTTCAGCTGAACGCAGCTAGCCCTTCAGGACTCTCAGGAGCTCCTCGACAGAGCAGCGATCAGCAGCTTAGGAGAGAGCCTTCAGGAGTCAGGTTTCCATCGGTCGGTACGGTACGACAGCTGAGAGCTCTAGCTAGCCCTTCAGCAGCTAGCTGACTAGGACGACGACTAGCGTACGTACTGACTTAGAGCGACTCTCTCCTAGGACTAGCTAGCTAGGAGCTCTCTCAGACGCTAGAGCTGACCTCTCTCAGAGAGCTAGCTAGCGCGAGT

7. Triplet code CTG GCC TAG AAT CCA ATG
Called “codons” – code for amino acids
Start of gene indicated with a Start codon – ATG – codes for Methionine (M)
End of gene indicated with a Stop codon –(TAG, TAA, TGA)

8. double helix
anti-parallel

9. 5’ 3’ A C T G T A G C T 3’ 5’

10. 5ATGGGGCCATTCGAATCTAG…. lotsandlotsandlotsofbases…
5ATGGGGCCATTCGAATCTAG….lotsandlotsandlotsofbases….AGCGATTATTTGGCTAGACAGTAA3
3AATGGGCCATTCGAATCTAG….lotsandlotsandlotsofbases….AGCGATTATTTGGCTAGACAGGTA5

11. DNA RNA Protein Protein TRANSCRIPTION TRANSLATION ATTCCGATGGGT
TAAGGCATCCCA
AUUCCGAUGGGU
TRANSCRIPTION
TRANSLATION

12. “curating” the genes… introns and exons and splicing….
your job…
“curating” the genes…
introns and exons and splicing….

13. RUDOLPHXXXXTHEXXXREDXXXXXXXXNOSEDXXXXXXXXXXREINXXXDEERXXXXXXXXXXXHADAXXXXVERYXXXSHINYXXXXNOSE
GENE (DNA)
RUDOLPHXXXXTHEXXXREDXXXXXXXXNOSEDXXXXXXXXXXREINXXXDEERXXXXXXXXXXXHADAXXXXVERYXXXSHINYXXXXNOSE
Transcribed mRNA
edited mRNA (introns removed
RUDOLPHTHEREDNOSEDEAREDREINDEERHADAVERYSHINYNOSE

14. Intron
Exon
5’UTR
CDS
CDS
CDS
3’UTR
Start codon (ATG) here
Stop codon here

15. Whipworm genome 3 chromosomes 80,000,000 base pairs (i.e. DNA letters)
In fragments (chromosomes 1 and 3 not fully reconstructed)
e.g. chromosome 1 in 7 fragments (aka “scaffolds”)
OHS allocated a scaffold to annotate

16. What next?
Dr W will send with introduction to Apollo – watch the training videos!
Once you’ve done this, collect your avatar and log-in details from Dr W
You can then log into Apollo and try the training exercises
Learn by doing!!!!!
You may prefer to work in pairs or small groups