1. The Microscope

2. The Parts of a Microscope

3. Ocular Lens
Body Tube
Nose Piece
Arm
Objective
Lenses
Stage
Stage
Clips
Coarse Adj.
Diaphragm
Fine Adjustment
Light Source
Base
Skip to Magnification Section

4. Body Tube
The body tube holds the objective lenses and the ocular lens at the proper distance
Diagram

5. Nose Piece
The Nose Piece holds the objective lenses and can be turned to increase the magnification
Diagram

6. Objective Lenses
The Objective Lenses increase magnification (usually from 10x to 40x)
Diagram

7. Stage Clips
These 2 clips hold the slide/specimen in place on the stage.
Diagram

8. Diaphragm
The Diaphragm controls the amount of light on the slide/specimen
Turn to let more light in or to
make dimmer.
Diagram

9. Light Source
Projects light upwards through the diaphragm, the specimen and the lenses
Some have lights, others have mirrors where you must move the mirror to reflect light
Diagram

10. Ocular Lens/Eyepiece
Magnifies the specimen image
Diagram

11. Arm
Used to support the microscope when carried. Holds the body tube, nose piece and objective lenses
Diagram

12. Stage
Supports the slide/specimen
Diagram

13. Coarse Adjustment Knob
Moves the stage up and down (quickly) for focusing your image
Diagram

14. Fine Adjustment Knob
This knob moves the stage SLIGHTLY to sharpen the image
Diagram

15. Base
Supports the microscope
Diagram

16. Magnification

17. Fill in the table on your worksheet.
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Fill in the table on your worksheet.

18. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

19. Caring for a Microscope
Clean only with a soft cloth/tissue
Make sure it’s on a flat surface
Don’t bang it
Carry it with 2 HANDS…one on the arm and the other on the base

20. Carry a Microscope Correctly

21. Using a Microscope Start on the lowest magnification
Don’t use the coarse adjustment knob on high magnification…you’ll break the slide!!!
Place slide on stage and lock clips
Adjust light source (if it’s a mirror…don’t stand in front of it!)
Use fine adjustment to focus

22. Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place.
2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!
Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!
4 – When you are done, turn off the microscope and clean up or put away the slides you used.

23. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
VIDEO
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

24. References http://www.cerebromente.org.br/n17/history/neurons1_i.htm
Google Images

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