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Fig. 1. Mitochondrial internalization in cardiomyocytes.

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Presentation on theme: "Fig. 1. Mitochondrial internalization in cardiomyocytes."— Presentation transcript:

1 Fig. 1. Mitochondrial internalization in cardiomyocytes.
Mitochondrial internalization in cardiomyocytes. (A) Representative florescent micrographs of isolated rat liver mitochondria labeled with pHrodo co-incubated with cardiomyocytes for 4 hour (middle panel) or 24 hours (right panel). Control cardiomyocytes (no mitochondria) are shown in the left panel. In all images the blue stain is DAPI; red, pHrodo labeled mitochondria, green is 488 phalloidin (f-actin). Scale bars are 25 µm. Results show internalization of transplanted mitochondria at 4 and 24 hours. (B) ATP content in cardiomyocytes: ATP content (nmol/103 cells) in control, no mitochondria (white bars) and cardiomyocytes co-incubated with mitochondria (black bars). (C) Mitochondrial uptake at 1, 4 and 24 hour co-incubation. The mean±sd for n = 5 experiments is shown. Results indicate that ATP content is significantly increased (p<0.05) in cardiomyocytes following 24 hours co-incubation with liver mitochondria. ATP standard luminescence; R2 =  Statistical differences at p<0.05 vs. control is shown as *. Christina A. Pacak et al. Biology Open 2015;bio © Published by The Company of Biologists Ltd

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