1. PATJ is a common cellular target for the adenovirus E4-ORF1 and high-risk HPV type 18 E6 oncoproteins.
PATJ is a common cellular target for the adenovirus E4-ORF1 and high-risk HPV type 18 E6 oncoproteins. (A) PATJ PDZ8 mediates binding to E4-ORF1. COS7 cells on 6 cm dishes were lipofected with pRK5-myc-PATJ-wt, -ΔPDZ6, or -ΔPDZ8 (3 μg). Extracts (200 μg protein) of RIPA buffer-lysed cells were subjected to pulldown reactions with the indicated GST-E4-ORF1 fusion protein, and recovered proteins were immunoblotted with Myc antibody (left panel). An immunoblot assay of cell extracts with Myc antibody shows 3/40th of the input protein for each reaction (right panel). The control lane represents extracts from cells lipofected with pRK5-myc plasmid (3 μg). Vertical lines indicate boundary between spliced lanes from the same gel. (B) PATJ binds E4-ORF1 and the high-risk HPV-18 E6 oncoprotein. COS7 cells on 6 cm dishes were lipofected with pRK5-myc-PATJ (3 μg). Extracts (160 μg protein) of RIPA buffer-lysed cells were subjected to pulldown reactions with the indicated GST fusion proteins, and recovered proteins were immunoblotted with Myc antibody. An immunoblot assay of cell extracts with the Myc antibody shows 1/16 of the input protein for each reaction (rightmost lane). 18E6, HPV-18 E6; 11E6, HPV-11 E6.
Isabel J. Latorre et al. J Cell Sci 2005;118:
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