1. Geno- and phenotyping of selected keratinocyte cultures before and after conditional β-catenin gene ablation by Adenovirus-encoded Cre-recombinase.
Geno- and phenotyping of selected keratinocyte cultures before and after conditional β-catenin gene ablation by Adenovirus-encoded Cre-recombinase. (A) PCR performed on genomic DNA of cultured mouse keratinocytes depicts complete recombination of β-cat+loxP into β-cat- alleles in cultures 1-3. (B) Western blot analysis of whole cell lysates was used to confirm lack of β-catenin expression in conditional null mutant keratinocytes. Plakoglobin was assessed on the same blot and used as a loading control. β-, deleted β-catenin allele; β+loxP, transgenic allele carrying two loxP sites;β +, wild-type allele; PG, plakoglobin.
Horst Posthaus et al. J Cell Sci 2002;115:
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