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Indirect fluorescence assay of N. gruberi cells.

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Presentation on theme: "Indirect fluorescence assay of N. gruberi cells."— Presentation transcript:

1 Indirect fluorescence assay of N. gruberi cells.
Indirect fluorescence assay of N. gruberi cells. (A) Cellular localisation of COPI in N. gruberi cells. Chicken anti-N.gruberi COPB antiserum (1:250; green) shows a discrete localisation in the cells, while DAPI stains the Naegleria nucleus and mitochondrial DNA. Differential interference contrast (DIC) images show the cells used for immunofluorescence. (B) Cellular localisation of Sec31 in N. gruberi cells. Rat anti-N.gruberi Sec31 antiserum (1:250; red) shows a discrete localisation in the cells, while DAPI stains the Naegleria nucleus and mitochondrial DNA. DIC images show the cells used for immunofluorescence. (C) Cellular localisation of COPI and Sec31 in N. gruberi cells. Chicken anti-N.gruberi COPB antiserum (green) shows a discrete localisation in the cells that is not co-localised with the rat anti-N.gruberi Sec31 antiserum (red), while DAPI stains the Naegleria nucleus and mitochondrial DNA. DIC images show the the cells used for immunofluorescence. The experiment was performed three times in six replicates. Scale bars: 10 μm. Emily K. Herman et al. J Cell Sci 2018;131:jcs213306 © Published by The Company of Biologists Ltd

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