1. Effect of YopM on the transcription of pro-inflammatory cytokines in HL60-derived macrophages after stimulation with LPS. HL60 cells differentiated into macrophages were pre-incubated with recombinant YopM and YopM87-C (25 μg/ml, each) for 3 hours before stimulation with 1 μg/ml LPS for 16 hours.
Effect of YopM on the transcription of pro-inflammatory cytokines in HL60-derived macrophages after stimulation with LPS. HL60 cells differentiated into macrophages were pre-incubated with recombinant YopM and YopM87-C (25 μg/ml, each) for 3 hours before stimulation with 1 μg/ml LPS for 16 hours. After RNA isolation and cDNA construction, the mRNA levels of TNFα, IL-1β, IL-10, IL-12p35, IL-15 and IL-18 were assessed by qRT-PCR. All data were obtained using SYBR green and cytokine-specific primers. The mRNA level of medium-treated cells was set as 100%. Data represent means and s.d. of at least three independent experiments, each performed in duplicate. The asterisk (*) indicates significant differences (P<0.05) in the YopM-dependent reduction of LPS stimulation of pro-inflammatory cytokines.
Christian Rüter et al. J Cell Sci 2010;123:
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