1. Generation of iTS cells via ES cells carrying an inducible Cdx2 transgene.
Generation of iTS cells via ES cells carrying an inducible Cdx2 transgene. (A) Southern blot analysis of ES clones carrying an inducible Cdx2 transgene, as detected by Cdx2-exon 1 probe. Four independent clones were tested. (B) Immunofluorescence analysis for Oct4, Nanog, and Cdx2. Tx, tamoxifen. (C) Quantitative RT-PCR analysis for Oc4, Nanog, and Cdx2 mRNA. Levels are normalized to β-actin levels, with ES level set to 1. Mean ± 1 SD is shown. n = 3 biological replicates. (D) iTS cells form TS-like colonies and giant cells (arrows) typically seen in the trophoblast lineage.
Jennifer A. Erwin et al. Genetics 2012;192:
Copyright © 2012 by the Genetics Society of America