1. Erythroid progenitor capacity of Epo transgenic mice.
Erythroid progenitor capacity ofEpotransgenic mice. Tissue was collected, processed through a 40-μm strainer and placed into MethoCult (A) Spleen cells were plated at a concentration of 1×105 cells per MethoCult dish and counted 2 days later for CFU-E colonies. (B) Bone marrow cells were plated at 1×104 cells per dish and counted 2 days later for CFU-E colonies. (C) Spleen cells were plated at a concentration of 1x105 cells per MethoCult dish and counted 4 days later for BFU-E colonies. (D) Bone marrow cells were plated at a 1×104 cells per dish and counted 4 days later for BFU-E colonies.
Brandon M. Zeigler et al. Dis. Model. Mech. 2010;3:
©2010 by The Company of Biologists Limited