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Fig. 2. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11.

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Presentation on theme: "Fig. 2. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11."— Presentation transcript:

1 Fig. 2. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11 immunoprecipitate and IgG and no antibody control immunoprecipitates for MRE11 (B,C) K562 cells were treated with 100 µM etoposide for two hours prior to embedding in agarose on microscope slides. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11 immunoprecipitate and IgG and no antibody control immunoprecipitates for MRE11 (B,C) K562 cells were treated with 100 µM etoposide for two hours prior to embedding in agarose on microscope slides. The slides were incubated with various immunoprecipitates, the fluorescence levels for the topoisomerase II covalent complexes remaining after the incubations were measured, the fluorescence value for each nucleus was determined and normalised to the mean of the positive control incubated in buffer (100%). These are shown as percentage of FITC signal remaining on a slide after incubation with MRE11 IP  =  eluted components from MRE11 IP; Depl MRE11 IP  =  immunodepleted MRE 11 IP eluate, Ig cont IP  =  IgG control IP, No Ab cont IP  =  control IP without antibody. ***  =  p value < Ka Cheong Lee et al. Biology Open 2012;bio © Published by The Company of Biologists Ltd

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