1. Genetic suppression of NNS and FACT by set1∆ and jhd2∆ dependent and independent of H3K4me.
Genetic suppression of NNS and FACT by set1∆ and jhd2∆ dependent and independent of H3K4me. Plate spot assays (as described in Figure 1) were used to compare the growth of the indicated strains. Isogenic strains were constructed through genetic crosses with H3K4A and H3K4R substitution mutants from the Dharmacon Non Essential Histone H3 & H4 Mutant Collection. (A) Genetic interactions of set1∆, H3K4A, and H3K4R with pob3-7. (B) Genetic interactions of jhd2∆ and H3K4R with nab3-11. Growth of two independent isolates of each genotype is shown. (C) Genetic interactions of jhd2∆ and H3K4R with pob3-7. (D) Diploid strains containing csf1∆, set1∆, and either H3K4A or H3K4R were sporulated and dissected onto YPD medium. A representative field of the haploid ascospores recovered and their respective genotypes are shown.
Kwan Yin Lee et al. G3 2018;8:
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