1. Patient and control ONS cells had very large differences in gene expression.
Patient and control ONS cells had very large differences in gene expression. (A) Example of neurospheres forming in vitro in serum-free medium containing EGF and FGF2. (B) Examples of adherent ONS cells after passage of dissociated neurospheres, in serum-containing medium. Scale bar: 100 μm. (C) Examples of flow cytometric plots quantifying the numbers of cells (y-axis) against fluorescence intensity for each cell (x-axis) for each of the antigens indicated. Shaded curve shows cells labeled with an antibody to the antigen indicated. Open curve shows cells labeled with an isotype control antibody. (D) Positive cells calculated as the mean percentage of antibody-labeled cells with fluorescence intensity greater than the most fluorescent isotype control (n=9 or 10). (E) Rates of proliferation of patient cells (dashed line) and control cells (continuous line), expressed as a fold-change from the number at 24 hours (y-axis). (F) Responses of patient and control ONS cells in eight cell function assays: patient (shaded bars) and control (open bars). The response of each ONS cell line is normalized against the mean of the control cells for each assay (n=10). (G) Unsupervised cluster analysis of differentially expressed genes sorts the ONS cells into patients and controls. Rows are genes. Columns are individuals. The level of gene expression is indicated by the tone of the lines from overexpressed (yellow) to underexpressed (blue). Data in D-F indicate mean ± s.e.m.
Greger Abrahamsen et al. Dis. Model. Mech. 2013;6:
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