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Characterization of Col1a1FRT-STOP-FRT-Cre-ER-T2 mice.

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Presentation on theme: "Characterization of Col1a1FRT-STOP-FRT-Cre-ER-T2 mice."— Presentation transcript:

1 Characterization of Col1a1FRT-STOP-FRT-Cre-ER-T2 mice.
Characterization of Col1a1FRT-STOP-FRT-Cre-ER-T2 mice. (A) Immunofluorescence of tissues from 6-month-old Col1a1FRT-STOP-FRT-Cre-ER-T2; Rosa26mTmG/+ mice (n=2) without tamoxifen treatment show widespread tdTomato expression and no eGFP expression. (B) Immunofluorescence of tissues collected 30 days after one dose of intraperitoneal tamoxifen in 6-month-old mice (n=2) continue to show widespread tdTomato expression and no eGFP expression, indicating that the STOP cassette prevents expression of Cre-ERT2 in the absence of FlpO-mediated recombination and excision of the STOP cassette. Scale bars: 50 µm. Minsi Zhang, and David G. Kirsch Dis. Model. Mech. 2015;8: © Published by The Company of Biologists Ltd

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