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Antibody to very late activation antigen 4 prevents interleukin-5–induced airway hyperresponsiveness and eosinophil infiltration in the airways of guinea.

Published byMarguerite Lavallée Modified over 5 years ago

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Presentation on theme: "Antibody to very late activation antigen 4 prevents interleukin-5–induced airway hyperresponsiveness and eosinophil infiltration in the airways of guinea."— Presentation transcript:

1 Antibody to very late activation antigen 4 prevents interleukin-5–induced airway hyperresponsiveness and eosinophil infiltration in the airways of guinea pigs  Aletta D. Kraneveld, PhDa, Ingrid Van Arka, Henk J. Van Der Lindea, Dilniya Fattah, PhDb, Frans P. Nijkamp, PhDa, Antoon J.M. Van Oosterhout, PhDa  Journal of Allergy and Clinical Immunology  Volume 100, Issue 2, Pages (August 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 Fig. 1 Increase in RL after intravenous administration of histamine to guinea pigs 1 day after intranasal treatment with 1 μg IL5 (n = 7, closed bars) or with vehicle (n = 7, open bars). Results presented as mean ± SEM. *p < 0.05 compared with vehicle-treated animals for all histamine dosages studied. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 Fig. 2 Increase in RL after intravenous administration of histamine to guinea pigs treated: (top) with isotype-matched control antibody, 1E6: 2.5 mg/kg or (bottom) with antibody to VLA-4, HP1/2: 2.5 mg/kg, and treated intranasally with vehicle (open bars) or 1 μg IL5 (closed bars). IL5 or vehicle was administered twice 1 day (9 am and 4 pm) before experiment. Monoclonal antibodies were injected 1 hour before each IL5 or vehicle treatment. N = 5 to 7 animals per group. Results presented as mean ± SEM. *p < 0.05 compared with vehicle-treated animals for all histamine dosages studied. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 Fig. 2 Increase in RL after intravenous administration of histamine to guinea pigs treated: (top) with isotype-matched control antibody, 1E6: 2.5 mg/kg or (bottom) with antibody to VLA-4, HP1/2: 2.5 mg/kg, and treated intranasally with vehicle (open bars) or 1 μg IL5 (closed bars). IL5 or vehicle was administered twice 1 day (9 am and 4 pm) before experiment. Monoclonal antibodies were injected 1 hour before each IL5 or vehicle treatment. N = 5 to 7 animals per group. Results presented as mean ± SEM. *p < 0.05 compared with vehicle-treated animals for all histamine dosages studied. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 Fig. 3 Total number of eosinophils (× 105 cells) in BAL fluid of IL5 or vehicle-treated animals, which were pretreated with either anti-VLA-4 mAb (HP1/2: 2.5 mg/kg intravenously) or the isotype-matched mAb (1E6: 2.5 mg/kg intravenously). IL5 or vehicle were administered twice 1 day (9 am and 4 pm) before experiment. Monoclonal antibodies were injected 1 hour before each IL5 or vehicle treatment. Results presented as medians and individual values. N = 5 to 7 animals per group. *p < 0.05 compared with vehicle-treated animals. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 Fig. 4 Correlation between neutrophil numbers in BAL fluid and RL induced by a dose of 20 μg/kg histamine: in control animals (open symbols) and IL5-treated animals (closed symbols) after pretreatment with isotype-matched control antibody, 1E6: 2.5 mg/kg (circles) or with antibody to VLA-4, HP1/2: 2.5 mg/kg (squares). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

7 Fig. 5 Correlation (top) between eosinophil numbers in BAL fluid and lung resistance induced by a dose of 20 μg/kg histamine and (bottom) between EPO activity in cell-free BAL fluid and number of eosinophils in BAL fluid: in control animals (open symbols) and IL5-treated animals (closed symbols) after pretreatment with isotype-matched control antibody, 1E6: 2.5 mg/kg (circles) or with antibody to VLA-4, HP1/2: 2.5 mg/kg (squares). Correlations in both graphs were significant: p < 0.01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

8 Fig. 5 Correlation (top) between eosinophil numbers in BAL fluid and lung resistance induced by a dose of 20 μg/kg histamine and (bottom) between EPO activity in cell-free BAL fluid and number of eosinophils in BAL fluid: in control animals (open symbols) and IL5-treated animals (closed symbols) after pretreatment with isotype-matched control antibody, 1E6: 2.5 mg/kg (circles) or with antibody to VLA-4, HP1/2: 2.5 mg/kg (squares). Correlations in both graphs were significant: p < 0.01. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

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