Download presentation
Presentation is loading. Please wait.
Published byRudolf Somogyi Modified over 5 years ago
1
Polymorphism T→C (−34 bp) of gene CYP17 promoter in Greek patients with polycystic ovary syndrome
Evanthia Diamanti-Kandarakis, M.D., Ph.D., Michael I Bartzis, B.Sc., Evangelia D Zapanti, M.D., Ph.D., Giovanna G Spina, M.D., Fanny A Filandra, M.D., Thomais C Tsianateli, M.D., Angeliki T Bergiele, M.D., Chryssa R Kouli, M.D. Fertility and Sterility Volume 71, Issue 3, Pages (March 1999) DOI: /S (98)
2
FIGURE 1 Electrophoresis on a 3% agarose gel of the MspA1 I–digested polymerase chain reaction (PCR)-amplified 459-bp fragment from gene CYP17 promoter, which contains the site (−34bp) where the T→C transition occurs, creating the A2 allele. Lanes 2 and 3: Heterozygous A2 carriers. Lane 4: Individual homozygous for the A2 allele. Lane 5: Noncarrier of the A2 allele. Lane 6: Individual with intact PCR product (nonincubated with MspA1). Lane 8: pGL-2 basic DNA digested with MspA1 I. The sizes of the fragments were estimated using the ladder PhiX174 DNA Hae III digest (lanes 1 and 7) and Lambda DNA Hind III digest (lane 9). The gel was stained with ethidium bromide to permit visualization of DNA bands on UV irradiation. Fertility and Sterility , DOI: ( /S (98) )
3
FIGURE 2 Mean serum total T levels in the three CYP17 polymorphism genotype groups. There were 17, 29, and 4 individuals in the A1A1, A1A2, and A2A2 groups, respectively. Mean (SD) serum total T levels for the three groups were as follows: A1A1, 75.7 ± 32.2 ng/dL; A1A2, 77.8 ± 29.9 ng/dL; and A2A2, 87 ± 2.8 ng/dL. P = not significant (A1A1 versus A1A2, A1A1 versus A2A2, and A1A2 versus A2A2). Fertility and Sterility , DOI: ( /S (98) )
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.