1. Ondřej Zítka Datum konání: 6.11.2015
Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
Ondřej Zítka
Datum konání:
Investice do rozvoje vzdělávání

2. Background
Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids.

3. The Ultrafiltration of vesicles

4. Summary
The authors shows that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.

5. Investice do rozvoje vzdělávání
Acknowledgements:
Project:
Paper:
Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie (NANOTEAM) CZ.1.07/2.3/.00/
Nordin, J.Z., et al., Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine-Nanotechnology Biology and Medicine, (4): p
Investice do rozvoje vzdělávání