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Detection of micrometastases in histologically negative lymph nodes in esophageal cancer  James D Luketich, MD, Edmund S Kassis, BS, Sharon P Shriver,

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Presentation on theme: "Detection of micrometastases in histologically negative lymph nodes in esophageal cancer  James D Luketich, MD, Edmund S Kassis, BS, Sharon P Shriver,"— Presentation transcript:

1 Detection of micrometastases in histologically negative lymph nodes in esophageal cancer 
James D Luketich, MD, Edmund S Kassis, BS, Sharon P Shriver, PhD, Ninh T Nguyen, MD, Philip R Schauer, MD, Tracey L Weigel, MD, Samuel A Yousem, MD, Jill M Siegfried, PhD  The Annals of Thoracic Surgery  Volume 66, Issue 5, Pages (November 1998) DOI: /S (98)

2 Fig 1 Ethidium bromide gel demonstrating complementary deoxyribonucleic acid (cDNA) products of reverse transcriptase–polymerase chain reaction (RT-PCR) assay. The known size of the RT–PCR cDNA fragment of carcinoembryonic antigen (CEA) is 131 base pair (131 bp fragment). Lane 1 is the 100 base pair ladder; lane 2 is empty; lane 3 is a CEA–positive control (pc); lane 4 is a negative control (nc); and lanes 5 through 15 are lymph node samples. The results of histologic study are shown below each lane. (+ = histologically positive lymph node; − = histologically negative lymph node.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )


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