1. Fertility and Sterility
Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells 
Ali Salmassi, Ph.D., Andreas G Schmutzler, M.D., Lili Huang, M.D., Jürgen Hedderich, Diplom. Informatics, Walter Jonat, M.D., Liselotte Mettler, M.D. 
Fertility and Sterility 
Volume 81, Pages (March 2004)
DOI: /j.fertnstert

2. Figure 1
Immunocytochemical demonstration of G-CSF and its receptor (G-CSFR). (a) Granulocyte colony-stimulating factor in the cytoplasma of human follicular GCs. (b) Localization of G-CSFR on the surface of GCs. (c) Granulosa cells without the primary antibody as negative control. (d) Localization of G-CSFR on the surface of monocytes, isolated from blood as positive control. Immunoalkaline phosphatase reaction. Magnification, ×600.
Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )

3. Figure 2
Effect of increasing culture time on G-CSF secretion by granulosa cells (mean G-CSF levels, 95% confidence interval). Results from three experiments (five patients each) performed in triplicate (P<.05). Number of cells, 2 × 104.
Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )

4. Figure 3
In vitro RT-PCR of G-CSF and its receptor (G-CSFR). The lanes show the following. Lane a, The separation of a molecular weight marker; lanes b–d, Granulocyte colony-stimulating factor amplicon in human GCs, in human monocytes, and in human placenta as positive control; lanes e–g, Granulocyte colony-stimulating factor receptor amplicon in human GCs, in human monocytes, and in human placenta as positive control; lane h, Negative control omitting RT; lanes i–k, GAPDH amplicon in human GCs, human monocytes, and placenta.
Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )